Biodegradation of low concentration of monochloroacetic acid-degrading Bacillus sp. TW1 isolated from terengganu water treatment and distribution plant

• Main Authors: Zulkifly, Ahmad Hafiz, -, Roslan, -, D.D.b, -, Hamid
• Format: Article
• Language: English; English
• Published: 2010
• Subjects: TC Hydraulic engineering. Ocean engineering
• Online Access: http://eprints.unisza.edu.my/2884/1/FH02-FPBSM-14-00434.png; http://eprints.unisza.edu.my/2884/2/FH02-FPBSM-14-01038.jpg; http://eprints.unisza.edu.my/2884/

Haloacetic acids (HAAs) are group of chemicals formed due to disinfect ion by products that can be detected during chlorination and chloramination of processed drinking water. In this study, Bacillus sp. strain TW1 identified by morphological/biochemical and PCR-amplified 16S rRNA gene was isolated from Kuala Terengganu water treatment and distribution plant. TW1was isolated due to its ability to grow in low concentration of monochloroacetic acid (MCA) of 0.5 mM, 10 times lower than normal MCA as sole carbon and energy source. Bacterial cell culture was grown in liquid minimal medium, pH 6.5 at 30'C on rotary shaker (150 rpm). Degradation of monochloroacetic acid was detected by measuring the amount of chloride ion released in the liquid minimal medium. Strain TW1 degraded mon0<chloroacetic acid at best with maximum chloride ion released of 0.32 jumol Cl mL-1 using 0.5 mM MCA concentration . Current results demonstrated that this is the first reported study on low concentration of MCA degradation by Bacillus sp.TW1.© 20 10 Asian Network for Scientific Information