Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR
The aim of this study was to develop a methicillin-resistant Staphylococcus aureus (MRSA) detection method based on the melting temperature analysis profiling of S. aureus clinical isolates from three different hospitals in Malaysia. Simplex and duplex real-time PCR assay was used for the simultaneo...
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my-unisza-ir.27502022-09-13T04:42:30Z http://eprints.unisza.edu.my/2750/ Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR Suhaili, Zarizal Ali, Abd Manaf -, Johari -, S.A.b RL Dermatology The aim of this study was to develop a methicillin-resistant Staphylococcus aureus (MRSA) detection method based on the melting temperature analysis profiling of S. aureus clinical isolates from three different hospitals in Malaysia. Simplex and duplex real-time PCR assay was used for the simultaneous detection of nuc (species-specific) and mecA (methicillin-resistance) genes in a single SYBR Green Ireal-time PCR tube assay. Evaluations were based on the melting temperature (T m) analysis of the amplicons using 23 S. aureus clinical isolates including three ATCC S. aureus standard strains. Real-time PCR amplification products with melting peaks at 78.39 ± 0.4°C and 74.41 ± O .G C were detected for nuc and mecA genes, respectively. Each real-time PCR assay was completed within two hours. This rapid genotypic method is useful for the detection of resistant determinant (mecA) and identification of S. aureus (nuc) clinical isolates, thus benefiting patient therapy in hospitals.© 2008 Springer Science+Business Media 8.V. 2009 Article NonPeerReviewed image en http://eprints.unisza.edu.my/2750/1/FH02-FPBSM-14-01229.jpg Suhaili, Zarizal and Ali, Abd Manaf and -, Johari and -, S.A.b (2009) Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR. World Journal of Microbiology and Biotechnology. pp. 253-258. ISSN 09593993 |
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RL Dermatology Suhaili, Zarizal Ali, Abd Manaf -, Johari -, S.A.b Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR |
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The aim of this study was to develop a methicillin-resistant Staphylococcus aureus (MRSA) detection method based on the melting temperature analysis profiling of S. aureus clinical isolates from three different hospitals in Malaysia. Simplex and duplex real-time PCR assay was used for the simultaneous detection of nuc (species-specific) and mecA (methicillin-resistance) genes in a single SYBR Green Ireal-time PCR tube assay. Evaluations were based on the melting temperature (T m) analysis of the amplicons using 23 S.
aureus clinical isolates including three ATCC S. aureus standard strains. Real-time PCR amplification products with melting peaks at 78.39 ± 0.4°C and 74.41 ± O .G C were detected for nuc and mecA genes, respectively. Each real-time PCR assay was completed within two hours. This rapid genotypic method is useful for the detection of resistant determinant (mecA) and identification of S. aureus (nuc) clinical isolates, thus benefiting patient therapy in hospitals.© 2008 Springer Science+Business Media 8.V. |
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Article |
author |
Suhaili, Zarizal Ali, Abd Manaf -, Johari -, S.A.b |
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Suhaili, Zarizal Ali, Abd Manaf -, Johari -, S.A.b |
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Suhaili, Zarizal |
title |
Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR |
title_short |
Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR |
title_full |
Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR |
title_fullStr |
Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR |
title_full_unstemmed |
Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR |
title_sort |
detection of malaysian methicillin-resistant staphylococcus aureus (mrsa) clinical isolates using simplex and duplex real-time pcr |
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2009 |
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http://eprints.unisza.edu.my/2750/1/FH02-FPBSM-14-01229.jpg http://eprints.unisza.edu.my/2750/ |
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