Thymoquinone effects on dopamine level and lactate dehydrogenase activity in differentiated SH-SY5Y cells exposed to amphetamine

• Main Authors: Nor Hidayah, Abu Bakar, Nasir, Mohamad, Nurul Farah Aina, Md Fauzi, Liyana Hazwani, Mohd Adnan, Nor Suliana, Mustafa
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2019
• Subjects: Q Science (General); QD Chemistry
• Online Access: http://eprints.unisza.edu.my/2539/1/FH03-FP-19-31406.pdf; http://eprints.unisza.edu.my/2539/2/FH03-FP-19-29501.pdf; http://eprints.unisza.edu.my/2539/

Aims 4/ Background: Amphetamine (AT) is used to treat some medical conditions and also is highly abused. It is a potent central nervous system stimulant that is capable of producing damaging effects to the central dopaminergic pathway. Thymoquinone (TQ), an active compound of Nigella sativa is known for its cellular protective effects. The objectives of this study were to determine the ICso value of AT and TQ on differentiated 51-1-5\15Y neuronal cells and to evaluate the changes of dopamine (DA) levels and lactate dehydrogenase (LDH) activity in the cells exposed to AT after co-administering with TQ. Methodology: Differentiated 511-1-5Y5Y cells were grown in cell culture flask containing DM EM/F12 with 10% (v/v) fetal bovine serum and 1% (w/v) penicillin/streptomycin. Cell viability was counted to determine the IC50 value of AT and TQ. Serially diluted solutions of AT (7396, 3698, 1849, 925, 462, 231, 116 and 0 pM) and TQ (6090, 3045, 1523, 761, 381, 190, 95 and 0 OA) were added to the cells and incubated for 72 hours.Subsequently, 20 pi of 3-(4,5-dimethylthiazol-2-y1)2,5- diphenyltetrazolium bromide and 100 pi of DM50 were added. The DA levels and LDH activity were determined by using the ELISA and LDH activity assay kits respectively. Results: The ICso values of AT and TQ were determined to be 1596 OA and 926 pM respectively. Co-administration of 30 phi of TQ and 37 of AT demonstrated a significant increase in DA levels when compared to administration of AT alone (P5.0.05). There was also a significant decrease in LDH activity in the cells exposed to both AT and TQ compared to administration of AT alone (iPLO.001).Conclusion: These findings suggested that TQ has a role in maintaining the DA activity and also possesses a neuroprotective effect against cell damage after long term AT exposure.