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Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)

Abstract Klebsiella pneumoniae (K. pneumoniae) is one of the most important members of Klebsiella genus in Enterobacteriacae family, which is responsible for pneumonia (the destructive lung inflammation disease). Vegetables are known as source of contamination with K. pneumonia. Raw vegetables a...

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Main Author: John, Tang Yew Huat
Format: Article
Language:English
English
Subjects:
S Agriculture (General)
Online Access:http://eprints.unisza.edu.my/2439/1/FH02-FPBSM-14-01225.pdf
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spelling my-unisza-ir.24392021-08-21T08:31:50Z http://eprints.unisza.edu.my/2439/ Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) John, Tang Yew Huat S Agriculture (General) Abstract Klebsiella pneumoniae (K. pneumoniae) is one of the most important members of Klebsiella genus in Enterobacteriacae family, which is responsible for pneumonia (the destructive lung inflammation disease). Vegetables are known as source of contamination with K. pneumonia. Raw vegetables are usually consumed in salads and other dishes. The aim of this study was to investigate the occurrence of K. pneumoniae in raw vegetables marketed in Malaysia. Two hundred commonly used salad vegetables (lettuces, parsley, cucumber, tomato and carrot) from hypermarkets and wet markets were investigated for presence of K. pneumoniae using Most Probable Number-Polymerase Chain Reaction (MPN-PCR). K. pneumoniae was found to be significantly more frequent (100%) and (82.5%) in lettuce and cucumbers, respectively. K. pneumoniae contamination was lowest in carrot samples (30%). All samples were contaminated with K. pneumoniae ranging from <3 to 1100 MPN/g. Results showed the high health risk associated with consumption of raw vegetables. Article NonPeerReviewed text en http://eprints.unisza.edu.my/2439/1/FH02-FPBSM-14-01225.pdf image en http://eprints.unisza.edu.my/2439/2/FH02-FPBSM-14-01459.png John, Tang Yew Huat Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR). International food research journal. pp. 1757-1762. ISSN 19854668
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
John, Tang Yew Huat
Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
description Abstract Klebsiella pneumoniae (K. pneumoniae) is one of the most important members of Klebsiella genus in Enterobacteriacae family, which is responsible for pneumonia (the destructive lung inflammation disease). Vegetables are known as source of contamination with K. pneumonia. Raw vegetables are usually consumed in salads and other dishes. The aim of this study was to investigate the occurrence of K. pneumoniae in raw vegetables marketed in Malaysia. Two hundred commonly used salad vegetables (lettuces, parsley, cucumber, tomato and carrot) from hypermarkets and wet markets were investigated for presence of K. pneumoniae using Most Probable Number-Polymerase Chain Reaction (MPN-PCR). K. pneumoniae was found to be significantly more frequent (100%) and (82.5%) in lettuce and cucumbers, respectively. K. pneumoniae contamination was lowest in carrot samples (30%). All samples were contaminated with K. pneumoniae ranging from <3 to 1100 MPN/g. Results showed the high health risk associated with consumption of raw vegetables.
format Article
author John, Tang Yew Huat
author_facet John, Tang Yew Huat
author_sort John, Tang Yew Huat
title Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
title_short Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
title_full Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
title_fullStr Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
title_full_unstemmed Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
title_sort detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (mpn-pcr)
url http://eprints.unisza.edu.my/2439/1/FH02-FPBSM-14-01225.pdf
http://eprints.unisza.edu.my/2439/2/FH02-FPBSM-14-01459.png
http://eprints.unisza.edu.my/2439/
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score 13.211869

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