Investigation of the bismuth oxide nanoparticles on bystander effect in MCF-7 and hFOB 1.19 cells
This study aims to investigate the effect of bismuth oxide nanoparticles (Bi2O3 NPs) on the radiation induced bystander effect (RIBE) in MCF-7 and hFOB 1.19 cells line. The cells were irradiated with radiation doses of to 12 Gy using 6 MV photon beam in a single exposure. The irradiated cells&#...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/1938/1/FH03-FSK-20-37891.pdf http://eprints.unisza.edu.my/1938/ |
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| Summary: | This study aims to investigate the effect of bismuth oxide nanoparticles (Bi2O3 NPs) on the radiation induced
bystander effect (RIBE) in MCF-7 and hFOB 1.19 cells line. The cells were irradiated with radiation doses of to 12 Gy
using 6 MV photon beam in a single exposure. The irradiated cells' culture media were transferred to non-irradiated
bystander cells hour post-irradiation. PrestoBlue assay was then performed in this experiment to assess the cells'
viability. Results of cell viability percentage in all bystander cell groups compared to the control showed no significant
differences (P > 0.05) for both MCF-7 and hFOB 1.19 cell lines. The test also revealed no radiation dose dependencies
for all bystander cells groups. The present study demonstrated that MCF-7 and hFOB 1.19 bystander cells were able to
proliferate (> 80%) after hours incubation with irradiated-cell conditioned medium (ICCM) treated with Bi2 O3 NPs.
In summary, the use of Bi2O3 NPs for radio sensitization in radiotherapy is safe and do not increase the RIBE responses
in non-targeted cells. RIBE remained as one of the most crucial factors that need to be address and considered for the
application of nanoparticles as radiosensitizers in radiotherapy. |
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