Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining

The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang’s liver cell lines by usi...

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主要な著者: Wan Haifa Haryani Wan Omar,, Shahrul Hisham Zainal Ariffin,, Zaidah Zainal Ariffin,, Muhd Fauzi Safian,, Sahidan Senafi,, Rohaya Megat Abdul Wahab,
フォーマット: 論文
言語:English
出版事項: Universiti Kebangsaan Malaysia 2010
オンライン・アクセス:http://journalarticle.ukm.my/7437/1/26_Ayiesah.pdf
http://journalarticle.ukm.my/7437/
http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
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spelling my-ukm.journal.74372016-12-14T06:44:05Z http://journalarticle.ukm.my/7437/ Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab, The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang’s liver cell lines by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Ethanol extracts from eight Piperaceae families were selected randomly. Results showed that all eight spesies (P. sarmentosum, P. ramifilum, P. paucistigmum, P. betle, P. macronatum, P. ridleyi, P. magnibaccum and P. miniatum) showed cytotoxicity activity with Piper sarmentosum exhibit higher cytotoxicity activity with the IC50 value at 12.5 μg/mL. On the other hand, there were no cytotoxicity activity of Chang’s cell that could be induced by the ethanol extracts because the IC50 values for non-malignant Chang’s cell were greater than 30 μg/mL. Viability analysis using trypan blue showed that P. sarmentosum ethanol extract produced significant (p<0.05) decreased of HepG2 cells as compared to control (p<0.05). In conclusion, all ethanol extracts selected randomly from Piperaceae family showed cytotoxic using MTT assay. Viability analysis using trypan blue staining demonstrates that this type of analysis can become an alternative approach in determining cytotoxicity activity of cells in the presence of extracts. Universiti Kebangsaan Malaysia 2010-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7437/1/26_Ayiesah.pdf Wan Haifa Haryani Wan Omar, and Shahrul Hisham Zainal Ariffin, and Zaidah Zainal Ariffin, and Muhd Fauzi Safian, and Sahidan Senafi, and Rohaya Megat Abdul Wahab, (2010) Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining. Sains Malaysiana, 39 (6). pp. 941-949. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang’s liver cell lines by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Ethanol extracts from eight Piperaceae families were selected randomly. Results showed that all eight spesies (P. sarmentosum, P. ramifilum, P. paucistigmum, P. betle, P. macronatum, P. ridleyi, P. magnibaccum and P. miniatum) showed cytotoxicity activity with Piper sarmentosum exhibit higher cytotoxicity activity with the IC50 value at 12.5 μg/mL. On the other hand, there were no cytotoxicity activity of Chang’s cell that could be induced by the ethanol extracts because the IC50 values for non-malignant Chang’s cell were greater than 30 μg/mL. Viability analysis using trypan blue showed that P. sarmentosum ethanol extract produced significant (p<0.05) decreased of HepG2 cells as compared to control (p<0.05). In conclusion, all ethanol extracts selected randomly from Piperaceae family showed cytotoxic using MTT assay. Viability analysis using trypan blue staining demonstrates that this type of analysis can become an alternative approach in determining cytotoxicity activity of cells in the presence of extracts.
format Article
author Wan Haifa Haryani Wan Omar,
Shahrul Hisham Zainal Ariffin,
Zaidah Zainal Ariffin,
Muhd Fauzi Safian,
Sahidan Senafi,
Rohaya Megat Abdul Wahab,
spellingShingle Wan Haifa Haryani Wan Omar,
Shahrul Hisham Zainal Ariffin,
Zaidah Zainal Ariffin,
Muhd Fauzi Safian,
Sahidan Senafi,
Rohaya Megat Abdul Wahab,
Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
author_facet Wan Haifa Haryani Wan Omar,
Shahrul Hisham Zainal Ariffin,
Zaidah Zainal Ariffin,
Muhd Fauzi Safian,
Sahidan Senafi,
Rohaya Megat Abdul Wahab,
author_sort Wan Haifa Haryani Wan Omar,
title Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_short Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_full Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_fullStr Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_full_unstemmed Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_sort anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
publisher Universiti Kebangsaan Malaysia
publishDate 2010
url http://journalarticle.ukm.my/7437/1/26_Ayiesah.pdf
http://journalarticle.ukm.my/7437/
http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
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score 13.250246