Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.

The effects of ammonium tartrate and glucose concentration on biomass, lipid and GLA accumulation in Cunninghamella sp. 2A1 were investigated using Response Surface Methodology (RSM). Cultivation was carried out in 250 mL shake flask containing 100 mL of nitrogen limiting medium (with various combin...

Full description

Saved in:
Bibliographic Details
Main Authors: Farhila Muhid,, Abdul Jalil Abdul Kader,, Wan Mohtar Wan Yusoff,, Othman Omar,, Aidil Abdul Hamid,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2010
Online Access:http://journalarticle.ukm.my/7430/1/26_Ayiesah.pdf
http://journalarticle.ukm.my/7430/
http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.7430
record_format eprints
spelling my-ukm.journal.74302016-12-14T06:44:03Z http://journalarticle.ukm.my/7430/ Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1. Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid, The effects of ammonium tartrate and glucose concentration on biomass, lipid and GLA accumulation in Cunninghamella sp. 2A1 were investigated using Response Surface Methodology (RSM). Cultivation was carried out in 250 mL shake flask containing 100 mL of nitrogen limiting medium (with various combinations of concentration of ammonium tartrate (1-3 g/L) and glucose (30-60 g/L) at 30°C and 250 rpm agitation for 120 h. The concentration of both compounds significantly affected the biomass, lipid and GLA yield (p<0.05), with the production of each of them being represented by quadratic models. Higher concentration of ammonium tartrate and glucose (2.99 and 59.33 g/L, respectively) was required for enhanced biomass production whereas low nitrogen content with excess glucose was otherwise favoured for lipid and GLA production. Ammonium tartrate and glucose concentration at 1 and 43 g/L, respectively were estimated by the model and proven to give the highest lipid production and GLA yield of 31.06 % (g/g biomass) and 4.15 ×10-2 (g/g lipid less biomass), respectively. Universiti Kebangsaan Malaysia 2010-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7430/1/26_Ayiesah.pdf Farhila Muhid, and Abdul Jalil Abdul Kader, and Wan Mohtar Wan Yusoff, and Othman Omar, and Aidil Abdul Hamid, (2010) Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1. Sains Malaysiana, 39 (6). pp. 891-899. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The effects of ammonium tartrate and glucose concentration on biomass, lipid and GLA accumulation in Cunninghamella sp. 2A1 were investigated using Response Surface Methodology (RSM). Cultivation was carried out in 250 mL shake flask containing 100 mL of nitrogen limiting medium (with various combinations of concentration of ammonium tartrate (1-3 g/L) and glucose (30-60 g/L) at 30°C and 250 rpm agitation for 120 h. The concentration of both compounds significantly affected the biomass, lipid and GLA yield (p<0.05), with the production of each of them being represented by quadratic models. Higher concentration of ammonium tartrate and glucose (2.99 and 59.33 g/L, respectively) was required for enhanced biomass production whereas low nitrogen content with excess glucose was otherwise favoured for lipid and GLA production. Ammonium tartrate and glucose concentration at 1 and 43 g/L, respectively were estimated by the model and proven to give the highest lipid production and GLA yield of 31.06 % (g/g biomass) and 4.15 ×10-2 (g/g lipid less biomass), respectively.
format Article
author Farhila Muhid,
Abdul Jalil Abdul Kader,
Wan Mohtar Wan Yusoff,
Othman Omar,
Aidil Abdul Hamid,
spellingShingle Farhila Muhid,
Abdul Jalil Abdul Kader,
Wan Mohtar Wan Yusoff,
Othman Omar,
Aidil Abdul Hamid,
Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
author_facet Farhila Muhid,
Abdul Jalil Abdul Kader,
Wan Mohtar Wan Yusoff,
Othman Omar,
Aidil Abdul Hamid,
author_sort Farhila Muhid,
title Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_short Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_full Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_fullStr Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_full_unstemmed Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_sort optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by cunninghamella sp. 2a1.
publisher Universiti Kebangsaan Malaysia
publishDate 2010
url http://journalarticle.ukm.my/7430/1/26_Ayiesah.pdf
http://journalarticle.ukm.my/7430/
http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
_version_ 1643737128388001792
score 13.214268