Ability of acanthamoeba cyst to excyst at different temperatures
This study was carried out to observe thermotolerance ability of Acanthamoeba spp. A total of 32 Acanthamoeba spp. isolates obtained from water taps, sinks, swimming pools and sea water were used. Trophozoites of Acanthamoeba spp. were inoculated onto non-nutrient agar (NNA) seeded with heat-kille...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit UKM
2011
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| Online Access: | http://journalarticle.ukm.my/3607/1/Ability_of_Acanthamoeba_Cyst_to_Excyst_at_Different_Temperatures.pdf http://journalarticle.ukm.my/3607/ http://www.fsk.ukm.my/jurnal/jilid%209%281%29%202011/Chap%207_terbitan%209.1%20%282011%29.pdf |
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| Summary: | This study was carried out to observe thermotolerance ability of Acanthamoeba spp. A total of 32 Acanthamoeba spp.
isolates obtained from water taps, sinks, swimming pools and sea water were used. Trophozoites of Acanthamoeba spp.
were inoculated onto non-nutrient agar (NNA) seeded with heat-killed Escherichia coli using aseptic technique and
incubated for 14 days at 30°C to obtain the cyst. The cysts were subcultured onto new agar plates for thermotolerance
test at 37°C and 42°C. The plates were observed until 96 hours after incubation for excystation of Acanthamoeba before being declared negative. Overall, 81.25% of samples were able to excyst at 37°C while 37.5% were able to excyst at 42°C. Thermotolerant Acanthamoeba is associated with high pathogenicity potential. |
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