Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha
Search for inhibitors to insect proteases is one of many strategies to control pests. Previous work has demonstrated successful purification of effective inhibitors from plant origin. Thus, the current study attempted to purify protease inhibitors from locally available medicinal plants. The study d...
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Penerbit Universiti Kebangsaan Malaysia
2022
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my-ukm.journal.217602023-06-21T06:08:31Z http://journalarticle.ukm.my/21760/ Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha Zazali Alias, Nora Asyikin Ramli, Search for inhibitors to insect proteases is one of many strategies to control pests. Previous work has demonstrated successful purification of effective inhibitors from plant origin. Thus, the current study attempted to purify protease inhibitors from locally available medicinal plants. The study demonstrated that the ethanolic extracts of Mimosa diplotricha leaves caused a significant 80% reduction in bovine trypsin activity. The inhibitory property of the proteinaceous nature of the extract was reconfirmed through qualitative analysis using the detection of trypsin inhibitors on the SDS-PAGE technique. The ammonium precipitated trypsin inhibitor was purified using Hi-Trap G25 and resolved into a single band with a molecular weight of approximately 20.8 kDa. By using the Dixon plot the competitive inhibitor has a Ki value of 2.16 × 10-4 mM. The purified protein inhibited the protease extract of Chrysomya megacephala at IC50 of 28 μg/mL. The results highlighted the presence of trypsin inhibitor in Mimosa diplotricha and its potential as a pest control agent. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21760/1/MAS%2020.pdf Zazali Alias, and Nora Asyikin Ramli, (2022) Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha. Malaysian Applied Biology, 51 (4). pp. 169-175. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
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Search for inhibitors to insect proteases is one of many strategies to control pests. Previous work has demonstrated successful purification of effective inhibitors from plant origin. Thus, the current study attempted to purify protease inhibitors from locally available medicinal plants. The study demonstrated that the ethanolic extracts of Mimosa diplotricha leaves caused a significant 80% reduction in bovine trypsin activity. The inhibitory property of the proteinaceous nature of the extract was reconfirmed through qualitative analysis using the detection of trypsin inhibitors on the SDS-PAGE technique. The ammonium precipitated trypsin inhibitor was purified using Hi-Trap G25 and resolved into a single band with a molecular weight of approximately 20.8 kDa. By using the Dixon plot the competitive inhibitor has a Ki value of 2.16 × 10-4 mM. The purified protein inhibited the protease extract of Chrysomya megacephala at IC50 of 28 μg/mL. The results highlighted the presence of trypsin inhibitor in Mimosa diplotricha and its potential as a pest control agent. |
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Zazali Alias, Nora Asyikin Ramli, |
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Zazali Alias, Nora Asyikin Ramli, Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha |
author_facet |
Zazali Alias, Nora Asyikin Ramli, |
author_sort |
Zazali Alias, |
title |
Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha |
title_short |
Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha |
title_full |
Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha |
title_fullStr |
Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha |
title_full_unstemmed |
Purification and partial characterisation of a protease inhibitor from Mimosa diplotricha |
title_sort |
purification and partial characterisation of a protease inhibitor from mimosa diplotricha |
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Penerbit Universiti Kebangsaan Malaysia |
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2022 |
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http://journalarticle.ukm.my/21760/1/MAS%2020.pdf http://journalarticle.ukm.my/21760/ https://jms.mabjournal.com/index.php/mab/index |
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