A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame
A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective electrode. The pH transducer was made from non-plasticized polyacrylate...
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Penerbit Universiti Kebangsaan Malaysia
2022
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Online Access: | http://journalarticle.ukm.my/20646/1/14.pdf http://journalarticle.ukm.my/20646/ https://www.ukm.my/jsm/malay_journals/jilid51bil9_2022/KandunganJilid51Bil9_2022.html |
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my-ukm.journal.206462022-12-06T09:21:50Z http://journalarticle.ukm.my/20646/ A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective electrode. The pH transducer was made from non-plasticized polyacrylate membrane containing a hydrogen ionophore and lipophilic salt. The immobilized CES enzyme catalyzed the enzymatic hydrolysis of aspartame to L-aspartic acid (L-Asp), L-phenylalanine and methanol. Potentiometric determination of aspartame concentration was performed by quantifying the hydrogen ion concentration produced from L-Asp. The potentiometric determination of aspartame exhibited good selectivity with near Nernstian response. The sensitivity of the biosensor was closed to the Nernstian value, i.e., 50-52 mV decade-1 with a dynamic linear response range from 10-5 to 10-2 M and detection limit approaching 10-6 M. The aspartame biosensor demonstrated good repeatability and reproducibility with relative standard deviation (RSD) of 1.9% and 1.6%, respectively (n=3). The potentiometric aspartame biosensor was demonstrated to be reliable for determining aspartame content in sweetener samples and was comparable to the conventional high-performance liquid chromatography (HPLC) method for aspartame analysis. Penerbit Universiti Kebangsaan Malaysia 2022-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20646/1/14.pdf Noor Izaanin Ramli, and Lee, Yook Heng and Tan, Ling Ling (2022) A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame. Sains Malaysiana, 51 (9). pp. 2913-2924. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil9_2022/KandunganJilid51Bil9_2022.html |
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A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded
poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective
electrode. The pH transducer was made from non-plasticized polyacrylate membrane containing a hydrogen ionophore
and lipophilic salt. The immobilized CES enzyme catalyzed the enzymatic hydrolysis of aspartame to L-aspartic
acid (L-Asp), L-phenylalanine and methanol. Potentiometric determination of aspartame concentration was performed
by quantifying the hydrogen ion concentration produced from L-Asp. The potentiometric determination of aspartame
exhibited good selectivity with near Nernstian response. The sensitivity of the biosensor was closed to the Nernstian
value, i.e., 50-52 mV decade-1 with a dynamic linear response range from 10-5 to 10-2 M and detection limit approaching
10-6 M. The aspartame biosensor demonstrated good repeatability and reproducibility with relative standard deviation
(RSD) of 1.9% and 1.6%, respectively (n=3). The potentiometric aspartame biosensor was demonstrated to be reliable for
determining aspartame content in sweetener samples and was comparable to the conventional high-performance liquid
chromatography (HPLC) method for aspartame analysis. |
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Article |
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Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling |
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Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
author_facet |
Noor Izaanin Ramli, Lee, Yook Heng Tan, Ling Ling |
author_sort |
Noor Izaanin Ramli, |
title |
A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
title_short |
A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
title_full |
A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
title_fullStr |
A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
title_full_unstemmed |
A simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
title_sort |
simple potentiometric biosensor based on carboxylesterase for the analysis of aspartame |
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Penerbit Universiti Kebangsaan Malaysia |
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2022 |
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http://journalarticle.ukm.my/20646/1/14.pdf http://journalarticle.ukm.my/20646/ https://www.ukm.my/jsm/malay_journals/jilid51bil9_2022/KandunganJilid51Bil9_2022.html |
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