Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies

• Main Authors: Tan, Wei Miao, Lau, Seng Fong, Sharifah Zakiah Syed Sulaiman,, Nor Shahira Solehah Umran,, Mokrish Ajat,, Rozaihan Mansor,, Puteri Azaziah Megat Abd-Rani,, Intan NurFatiha Shafie,, Ng, Angela Min Hwei, Norasfaliza Rahmad,
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2021
• Online Access: http://journalarticle.ukm.my/18302/1/13.pdf; http://journalarticle.ukm.my/18302/; https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/KandunganJilid50Bil11_2021.html

The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canin...