New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.

The entomopathogenic fungal genus Lecanicillium Gams and Zare (formerly classified as the species Verticillium lecanii) includes species that are highly pathogenic to many insect genera. In this study, we identified six Lecanicillium spp. isolated strains (designated as V1-V6) belonging to L. leca...

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Main Authors: Liu, Liang, Liu, Chunlai, Yan, Lili, Yang, Fan, Wang, Shuang, Jiang, Xifeng, Li, Xinmin
格式: Article
语言:English
出版: Penerbit Universiti Kebangsaan Malaysia 2021
在线阅读:http://journalarticle.ukm.my/17529/1/11.pdf
http://journalarticle.ukm.my/17529/
https://www.ukm.my/jsm/malay_journals/jilid50bil6_2021/KandunganJilid50Bil6_2021.html
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spelling my-ukm.journal.175292021-10-26T04:55:41Z http://journalarticle.ukm.my/17529/ New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp. Liu, Liang Liu, Chunlai Yan, Lili Yang, Fan Wang, Shuang Jiang, Xifeng Li, Xinmin The entomopathogenic fungal genus Lecanicillium Gams and Zare (formerly classified as the species Verticillium lecanii) includes species that are highly pathogenic to many insect genera. In this study, we identified six Lecanicillium spp. isolated strains (designated as V1-V6) belonging to L. lecanii (V1, V3 and V5) and L. attenuatum (V2, V4 and V6). In addition, these strains were used to obtain new strains via protoplast fusion, and nit mutants were used for protoplast selection. Genetic recombination of the hybrid strains was determined using the random amplified polymorphic DNA (RAPD) technique. We obtained nine stable fusant strains from 176 new hybrid strains, which were termed V12-10, V14-3, V16-4, V23-6, V25-8, V34-14, V36-5, V45-16 and V56-7. Morphological characteristics varied between the hybrid and parental strains. Genomic DNA analysis of the fusants also showed genetic recombination. The median lethal concentration (LC50) for the fusants were lower than that for parental strains, and the median survival time (LT50) for the fusants were reduced compared with that for parental strains. Thus, these results showed that we produced new, more virulent hybrid Lecanicillium spp. strains as biological control agents via intraspecific protoplast fusion. Penerbit Universiti Kebangsaan Malaysia 2021-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17529/1/11.pdf Liu, Liang and Liu, Chunlai and Yan, Lili and Yang, Fan and Wang, Shuang and Jiang, Xifeng and Li, Xinmin (2021) New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp. Sains Malaysiana, 50 (6). pp. 1639-1650. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil6_2021/KandunganJilid50Bil6_2021.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The entomopathogenic fungal genus Lecanicillium Gams and Zare (formerly classified as the species Verticillium lecanii) includes species that are highly pathogenic to many insect genera. In this study, we identified six Lecanicillium spp. isolated strains (designated as V1-V6) belonging to L. lecanii (V1, V3 and V5) and L. attenuatum (V2, V4 and V6). In addition, these strains were used to obtain new strains via protoplast fusion, and nit mutants were used for protoplast selection. Genetic recombination of the hybrid strains was determined using the random amplified polymorphic DNA (RAPD) technique. We obtained nine stable fusant strains from 176 new hybrid strains, which were termed V12-10, V14-3, V16-4, V23-6, V25-8, V34-14, V36-5, V45-16 and V56-7. Morphological characteristics varied between the hybrid and parental strains. Genomic DNA analysis of the fusants also showed genetic recombination. The median lethal concentration (LC50) for the fusants were lower than that for parental strains, and the median survival time (LT50) for the fusants were reduced compared with that for parental strains. Thus, these results showed that we produced new, more virulent hybrid Lecanicillium spp. strains as biological control agents via intraspecific protoplast fusion.
format Article
author Liu, Liang
Liu, Chunlai
Yan, Lili
Yang, Fan
Wang, Shuang
Jiang, Xifeng
Li, Xinmin
spellingShingle Liu, Liang
Liu, Chunlai
Yan, Lili
Yang, Fan
Wang, Shuang
Jiang, Xifeng
Li, Xinmin
New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.
author_facet Liu, Liang
Liu, Chunlai
Yan, Lili
Yang, Fan
Wang, Shuang
Jiang, Xifeng
Li, Xinmin
author_sort Liu, Liang
title New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.
title_short New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.
title_full New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.
title_fullStr New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.
title_full_unstemmed New hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi Lecanicillium spp.
title_sort new hybrid strains via intraspecific protoplast fusion of the entomopathogenic fungi lecanicillium spp.
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/17529/1/11.pdf
http://journalarticle.ukm.my/17529/
https://www.ukm.my/jsm/malay_journals/jilid50bil6_2021/KandunganJilid50Bil6_2021.html
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