Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice
Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological activities. This study aimed to evaluate the photoprotective...
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2021
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my-ukm.journal.174162021-09-14T03:08:15Z http://journalarticle.ukm.my/17416/ Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological activities. This study aimed to evaluate the photoprotective effect of pterostilbene on ultraviolet-B-induced BALB/c mice. Twenty-four female mice were randomised into four groups (n=6/group): vehicle control (VC); UVB irradiated only (UVB only); UVB irradiation treated with pterostilbene 10 mg/kg (PS10+UVB); (iv) UVB irradiation treated with pterostilbene 20 mg/kg (PS20+UVB). The PS treatments were given for 14 days, and UVB was given at dose 250 mJ/ cm2 on days 9, 11, and 13 of the treatment periods. The results showed that PS lessened redness and scaling on the skin of UVB-irradiated mice. The skinfold thickness and epidermal thickness in the PS-treated group were significantly reduced (p<0.05) in comparison with those in the UVB only group. The PS10 and PS20 groups (5.927 ± 0.354 and 5.660 ± 0.765 nmol/g, respectively) demonstrated significantly decreased MDA levels (P<0.05) relative to the UVB only group (13.343 ± 1.350 nmol/g). The GSH level in both PS10 (0.555 ± 0.020 µmol/mg) and PS20 (0.568 ± 0.055 µmol/mg) groups increased significantly (p<0.05) compared with that in the UVB only group (0.376 ± 0.025 µmol/mg). SOD activity in the PS20 group (1.388 ± 0.172 U/min/mg) increased significantly (p<0.05) compared with that in the UVB only group (0.561 ± 0.034 U/min/mg). Histological observation showed that PS reduced leukocyte infiltration and epidermal hyperplasia. Hence, oral PS may exert a photoprotective effect by acting as an anti-inflammatory and antioxidant agent on UVB-irradiated mice skin. Penerbit Universiti Kebangsaan Malaysia 2021-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17416/1/19.pdf Tava Shelan Nagapan, and Lim, Wenna Nallance and Ahmad Rohi Ghazali, and Dayang Fredalina Basri, (2021) Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice. Sains Malaysiana, 50 (5). pp. 1407-1414. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil5_2021/KandunganJilid50Bil5_2021.html |
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Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and
cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological
activities. This study aimed to evaluate the photoprotective effect of pterostilbene on ultraviolet-B-induced BALB/c
mice. Twenty-four female mice were randomised into four groups (n=6/group): vehicle control (VC); UVB irradiated
only (UVB only); UVB irradiation treated with pterostilbene 10 mg/kg (PS10+UVB); (iv) UVB irradiation treated with
pterostilbene 20 mg/kg (PS20+UVB). The PS treatments were given for 14 days, and UVB was given at dose 250 mJ/
cm2
on days 9, 11, and 13 of the treatment periods. The results showed that PS lessened redness and scaling on the
skin of UVB-irradiated mice. The skinfold thickness and epidermal thickness in the PS-treated group were significantly
reduced (p<0.05) in comparison with those in the UVB only group. The PS10 and PS20 groups (5.927 ± 0.354 and
5.660 ± 0.765 nmol/g, respectively) demonstrated significantly decreased MDA levels (P<0.05) relative to the UVB
only group (13.343 ± 1.350 nmol/g). The GSH level in both PS10 (0.555 ± 0.020 µmol/mg) and PS20 (0.568 ± 0.055
µmol/mg) groups increased significantly (p<0.05) compared with that in the UVB only group (0.376 ± 0.025 µmol/mg).
SOD activity in the PS20 group (1.388 ± 0.172 U/min/mg) increased significantly (p<0.05) compared with that in
the UVB only group (0.561 ± 0.034 U/min/mg). Histological observation showed that PS reduced leukocyte infiltration
and epidermal hyperplasia. Hence, oral PS may exert a photoprotective effect by acting as an anti-inflammatory and
antioxidant agent on UVB-irradiated mice skin. |
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Article |
author |
Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, |
spellingShingle |
Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
author_facet |
Tava Shelan Nagapan, Lim, Wenna Nallance Ahmad Rohi Ghazali, Dayang Fredalina Basri, |
author_sort |
Tava Shelan Nagapan, |
title |
Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
title_short |
Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
title_full |
Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
title_fullStr |
Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
title_full_unstemmed |
Pterostilbene supplementation inhibits early inflammatory response and oxidative stress in UVB-induced BALB/C mice |
title_sort |
pterostilbene supplementation inhibits early inflammatory response and oxidative stress in uvb-induced balb/c mice |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
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2021 |
url |
http://journalarticle.ukm.my/17416/1/19.pdf http://journalarticle.ukm.my/17416/ https://www.ukm.my/jsm/malay_journals/jilid50bil5_2021/KandunganJilid50Bil5_2021.html |
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13.160551 |