Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers
Genetic diversity and variation of three naturally distributed Rhododendron moulmainense populations in the Lam Vien Plateau of Vietnam were analyzed using ISSR and SCoT markers separately and data from the two types of markers were then combined to show the overall results. Based on combined data...
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my-ukm.journal.173832021-08-30T03:40:04Z http://journalarticle.ukm.my/17383/ Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers Luu, The Trung La, Anh Duong Tran, Van Tien Le, Ngoc Trieu Phi, Hong Hai Genetic diversity and variation of three naturally distributed Rhododendron moulmainense populations in the Lam Vien Plateau of Vietnam were analyzed using ISSR and SCoT markers separately and data from the two types of markers were then combined to show the overall results. Based on combined data, the genetic diversity parameters of the Tuyen Lam population were percentage of polymorphic bands (PPB) = 28.79%; genetic heterozygosity (He) = 0.1041; Shannon index (I) = 0.1552. The corresponding parameters were PPB = 37.88%; He = 0.1434; I = 0.2131 in the Hon Nga population, and PPB = 46.21%; He = 0.1857; I = 0.2709 in the Bidoup population. At the species level in Lam Dong province, the corresponding parameters were PPB = 62.12%; He = 0.1747; I = 0.2756. The average of pairwise genetic similarity coefficients among investigated individuals of Tuyen Lam population was 0.973, of Hon Nga population was 0.911, and of Bidoup population was 0.895. The genetic differentiation and the number of migrants per generation among investigated populations were GSTS = 0.1735 and Nm = 2.3824 individuals, respectively. Genetic distance between Tuyen Lam and Hon Nga populations was 0.0399, between Tuyen Lam and Bidoup populations were 0.0621, and Bidoup and Hon Nga populations were 0.0670. Data generated by both marker types and the combined revealed similar trends of genetic diversity levels among the studied populations. The SCoT data showed higher values of genetic diversity and population gene differentiation than did the ISSR data, but the latter showed higher gene differentiation among sampled individuals in the study populations. Comparing the values of Polymorphism Information Content (PIC) for discriminatory ability; Effective Multiplex Ratio (EMR) for several revealed loci and/or efficiency of the primer-marker system; Marker Index (MI) for the efficiency of used method; and Resolving Power (Rp) between used techniques indicated that discriminatory ability, efficiency, and ability of the ISSR primer combinations were higher but using SCoT marker system was more efficient than ISSR marker system for evaluating genetic diversity. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17383/1/49_05_05.pdf Luu, The Trung and La, Anh Duong and Tran, Van Tien and Le, Ngoc Trieu and Phi, Hong Hai (2020) Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers. Malaysian Applied Biology, 49 (5). pp. 41-52. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56 |
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Genetic diversity and variation of three naturally distributed Rhododendron moulmainense populations in the Lam Vien Plateau
of Vietnam were analyzed using ISSR and SCoT markers separately and data from the two types of markers were then
combined to show the overall results. Based on combined data, the genetic diversity parameters of the Tuyen Lam population
were percentage of polymorphic bands (PPB) = 28.79%; genetic heterozygosity (He) = 0.1041; Shannon index (I) = 0.1552.
The corresponding parameters were PPB = 37.88%; He = 0.1434; I = 0.2131 in the Hon Nga population, and PPB = 46.21%;
He = 0.1857; I = 0.2709 in the Bidoup population. At the species level in Lam Dong province, the corresponding parameters
were PPB = 62.12%; He = 0.1747; I = 0.2756. The average of pairwise genetic similarity coefficients among investigated
individuals of Tuyen Lam population was 0.973, of Hon Nga population was 0.911, and of Bidoup population was 0.895.
The genetic differentiation and the number of migrants per generation among investigated populations were GSTS = 0.1735
and Nm = 2.3824 individuals, respectively. Genetic distance between Tuyen Lam and Hon Nga populations was 0.0399,
between Tuyen Lam and Bidoup populations were 0.0621, and Bidoup and Hon Nga populations were 0.0670. Data generated
by both marker types and the combined revealed similar trends of genetic diversity levels among the studied populations. The
SCoT data showed higher values of genetic diversity and population gene differentiation than did the ISSR data, but the latter
showed higher gene differentiation among sampled individuals in the study populations. Comparing the values of
Polymorphism Information Content (PIC) for discriminatory ability; Effective Multiplex Ratio (EMR) for several revealed
loci and/or efficiency of the primer-marker system; Marker Index (MI) for the efficiency of used method; and Resolving
Power (Rp) between used techniques indicated that discriminatory ability, efficiency, and ability of the ISSR primer
combinations were higher but using SCoT marker system was more efficient than ISSR marker system for evaluating genetic
diversity. |
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Luu, The Trung La, Anh Duong Tran, Van Tien Le, Ngoc Trieu Phi, Hong Hai |
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Luu, The Trung La, Anh Duong Tran, Van Tien Le, Ngoc Trieu Phi, Hong Hai Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers |
author_facet |
Luu, The Trung La, Anh Duong Tran, Van Tien Le, Ngoc Trieu Phi, Hong Hai |
author_sort |
Luu, The Trung |
title |
Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers |
title_short |
Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers |
title_full |
Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers |
title_fullStr |
Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers |
title_full_unstemmed |
Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien Plateau, Vietnam revealed by ISSR and SCoT markers |
title_sort |
genetic diversity of naturally distributed rhododendron moulmainense hook. f. populations in lam vien plateau, vietnam revealed by issr and scot markers |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
publishDate |
2020 |
url |
http://journalarticle.ukm.my/17383/1/49_05_05.pdf http://journalarticle.ukm.my/17383/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56 |
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13.18916 |