Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression
Hydrogen peroxide (H2O2) and oxygen (O2), reactive oxygen species (ROS) that are formed during wound healing. Excessive exposure to H2O2 may lead to oxidative stress in cells. However, the low or moderate dosage of H2O2 might help in the wound healing process. AlamarBlueTM cell viability, scratch...
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Penerbit Universiti Kebangsaan Malaysia
2020
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Online Access: | http://journalarticle.ukm.my/17250/1/49_04_35.pdf http://journalarticle.ukm.my/17250/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
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my-ukm.journal.172502021-07-29T04:13:01Z http://journalarticle.ukm.my/17250/ Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression Nur Syakirah Mahamad Aziz, Johnson Stanslas, Nurul Huda Abd Kadir, Hydrogen peroxide (H2O2) and oxygen (O2), reactive oxygen species (ROS) that are formed during wound healing. Excessive exposure to H2O2 may lead to oxidative stress in cells. However, the low or moderate dosage of H2O2 might help in the wound healing process. AlamarBlueTM cell viability, scratch assay, and qRT-PCR analysis were done to evaluate cell viability, cell migration and gene expression of VEGF-α and TGF-α on the skin fibroblast cells treated with H2O2 (0 to 50 µM), respectively. Our results have shown that the fibroblast cells were significantly proliferated and migrated after treated with H2O2 (12.5 µM, 25 µM, and 50 µM) at 48h and 72h of exposure when compared to control. Furthermore, VEGF-α gene expression on treated fibroblast cells (50 µM) was found to be higher than control, whereas TGF-α gene expression was slightly lower than control. This result suggesting that cell proliferation and migration was possibly due to the activation of VEGF-α but not TGF-α gene. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17250/1/49_04_35.pdf Nur Syakirah Mahamad Aziz, and Johnson Stanslas, and Nurul Huda Abd Kadir, (2020) Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression. Malaysian Applied Biology, 49 (4). pp. 261-266. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
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Hydrogen peroxide (H2O2) and oxygen (O2), reactive oxygen species (ROS) that are formed during wound healing. Excessive
exposure to H2O2 may lead to oxidative stress in cells. However, the low or moderate dosage of H2O2 might help in the
wound healing process. AlamarBlueTM cell viability, scratch assay, and qRT-PCR analysis were done to evaluate cell viability,
cell migration and gene expression of VEGF-α and TGF-α on the skin fibroblast cells treated with H2O2 (0 to 50 µM),
respectively. Our results have shown that the fibroblast cells were significantly proliferated and migrated after treated with
H2O2 (12.5 µM, 25 µM, and 50 µM) at 48h and 72h of exposure when compared to control. Furthermore, VEGF-α gene
expression on treated fibroblast cells (50 µM) was found to be higher than control, whereas TGF-α gene expression was
slightly lower than control. This result suggesting that cell proliferation and migration was possibly due to the activation of
VEGF-α but not TGF-α gene. |
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Article |
author |
Nur Syakirah Mahamad Aziz, Johnson Stanslas, Nurul Huda Abd Kadir, |
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Nur Syakirah Mahamad Aziz, Johnson Stanslas, Nurul Huda Abd Kadir, Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression |
author_facet |
Nur Syakirah Mahamad Aziz, Johnson Stanslas, Nurul Huda Abd Kadir, |
author_sort |
Nur Syakirah Mahamad Aziz, |
title |
Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression |
title_short |
Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression |
title_full |
Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression |
title_fullStr |
Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression |
title_full_unstemmed |
Hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via VEGF-alpha AND TGF-alpha gene expression |
title_sort |
hydrogen peroxide promotes human skin fibroblast cells migration and proliferation at lower dose via vegf-alpha and tgf-alpha gene expression |
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Penerbit Universiti Kebangsaan Malaysia |
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2020 |
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http://journalarticle.ukm.my/17250/1/49_04_35.pdf http://journalarticle.ukm.my/17250/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
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