Grape seed proanthocyanidin improves lipopolysaccharide-induced myocardial toxicity in mice

• Main Authors: Liu, Xianchu, Li, Sha, Chen, Ruyan, Li, Yue, Liu, Ming, Peng, Huan
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2021
• Online Access: http://journalarticle.ukm.my/17168/1/6.pdf; http://journalarticle.ukm.my/17168/; https://www.ukm.my/jsm/malay_journals/jilid50bil4_2021/KandunganJilid50Bil4_2021.html

Lipopolysaccharide (LPS) is an important pathogenic factor for sepsis which results in cardiovascular diseases and even mortality. Proanthocyanidin, one of the main components in grape seed, has a wide range of biological activities in various diseases. The mouse model was established by intraperitoneal injection with LPS. Grape seed proanthocyanidin (GSP) was administered continuously for 8 days. Our results showed that pre-treatment of GSP dramatically ameliorated the level of creatine kinase (CK) and lactate dehydrogenase (LDH) to defend against LPS-induced myocardial toxicity (p<0.01). Moreover, pre-treatment of GSP significantly mitigated the expression of matrix metallopeptidase 2 (MMP-2) and matrix metallopeptidase 9 (MMP-9) to inhibit LPS-induced myocardial fibrosis (p<0.01). In addition, pre-treatment of GSP significantly increased superoxide dismutase (SOD) and catalase (CAT) activities to improve the level of malondialdehyde (MDA) and hydrogen peroxide (H2 O2 ) (p<0.01). Lastly, pre-treatment of GSP relieved tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) activities to prevent inflammatory responses (p<0.01).