Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4
This study reported the isolation and biochemical studies of extracellular lipase produced by a moderate halophilic bacterium, Halomonas meridiana BK-AB4. The estimated molecular weight of the lipase BK-AB4 is about 50 kDa. The study of metal ions effect to the enzyme activity revealed the strong...
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2020
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my-ukm.journal.160212020-12-18T02:12:36Z http://journalarticle.ukm.my/16021/ Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 Nanda, Galo Ayu Megga Hertadi, Rukman This study reported the isolation and biochemical studies of extracellular lipase produced by a moderate halophilic bacterium, Halomonas meridiana BK-AB4. The estimated molecular weight of the lipase BK-AB4 is about 50 kDa. The study of metal ions effect to the enzyme activity revealed the strong dependency towards the presence of Ca2+ ion. Further studies showed that the optimum pH of the enzyme was similar in the presence and the absence of Ca2+ ions. However, the presence of Ca2+ ions could improve the enzyme thermal stability as indicated by the shift of its optimum temperature from 45°C - 50°C. The addition of Ca2+ ions was also enhancing residual activity towards various inhibitors, such as SDS and PMSF up to 55% and 68%, respectively. Another interesting effect of the addition of Ca2+ ions were noted when various organic solvents were added to the enzyme solution in the ratio of 1:1. The activity of the enzyme increased up to 122% and 126% relative to the control when methanol and n-butanol were added, respectively. This study thus showed that the addition of Ca2+ ion not only improved the catalytic activity of lipase BK-AB4 but also made the enzyme more tolerable against various perturbations. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16021/1/49_02_05.pdf Nanda, Galo Ayu Megga and Hertadi, Rukman (2020) Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4. Malaysian Applied Biology, 49 (2). pp. 27-35. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56 |
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This study reported the isolation and biochemical studies of extracellular lipase produced by a moderate halophilic bacterium,
Halomonas meridiana BK-AB4. The estimated molecular weight of the lipase BK-AB4 is about 50 kDa. The study of metal
ions effect to the enzyme activity revealed the strong dependency towards the presence of Ca2+ ion. Further studies showed
that the optimum pH of the enzyme was similar in the presence and the absence of Ca2+ ions. However, the presence of Ca2+
ions could improve the enzyme thermal stability as indicated by the shift of its optimum temperature from 45°C - 50°C. The
addition of Ca2+ ions was also enhancing residual activity towards various inhibitors, such as SDS and PMSF up to 55%
and 68%, respectively. Another interesting effect of the addition of Ca2+ ions were noted when various organic solvents
were added to the enzyme solution in the ratio of 1:1. The activity of the enzyme increased up to 122% and 126% relative to
the control when methanol and n-butanol were added, respectively. This study thus showed that the addition of Ca2+ ion not
only improved the catalytic activity of lipase BK-AB4 but also made the enzyme more tolerable against various perturbations. |
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Article |
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Nanda, Galo Ayu Megga Hertadi, Rukman |
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Nanda, Galo Ayu Megga Hertadi, Rukman Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 |
author_facet |
Nanda, Galo Ayu Megga Hertadi, Rukman |
author_sort |
Nanda, Galo Ayu Megga |
title |
Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 |
title_short |
Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 |
title_full |
Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 |
title_fullStr |
Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 |
title_full_unstemmed |
Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4 |
title_sort |
effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria halomonas meridiana bk-ab4 |
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Penerbit Universiti Kebangsaan Malaysia |
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2020 |
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http://journalarticle.ukm.my/16021/1/49_02_05.pdf http://journalarticle.ukm.my/16021/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56 |
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