Successful and efficient DNA extraction from processed pangolin scales

• Main Authors: Xuan, Cai, Ye, Hao-Ting, Tian, En-Wei, Fang, Li, Chan, Li, Yang, Yao-Jun, Liu, Chun-Sheng, Zhi, Chao
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2019
• Online Access: http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf; http://journalarticle.ukm.my/13313/; http://www.ukm.my/jsm/malay_journals/jilid48bil3_2019/KandunganJilid48Bil3_2019.html

A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by d...