Acrylamide optical sensor based on hydrolysis using Bacillus sp. strain ZK34 containing amidase properties

• Main Authors: Chong, Yee May, Musa Ahmad,, Lee, Yook Heng, Norzila Kusnin,, Mohd Yunus Abdul Shukor,
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2017
• Online Access: http://journalarticle.ukm.my/11383/1/26%20Yee-May%20Chong.pdf; http://journalarticle.ukm.my/11383/; http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html

In this work, a new optical screening method for acrylamide was developed. Bacterial Bacillus sp. strain ZK 34 was used to hydrolyse acrylamide to the corresponding acid and ammonia. Nessler’s reagent was used to detect the produced ammonia and the yellow complex formed was treated as signal. Bacter...