Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris
Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. The recombinant vector, pPICZαA-243HPp was then linea...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/17110/1/molecular_cloning.pdf http://irep.iium.edu.my/17110/ http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/211/259 |
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