Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris
A cellobiohydrolase gene from the thermophilic fungus Humicola insolens ATCC 16454 was expressed in the methylotrophic yeast Pichia pastoris X-33, and the biochemical properties of the recombinant protein were characterised. The full-length cDNA of the cellobiohydrolase gene avi2 was cloned into t...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Malaysian Society of Applied Biology
2015
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| Online Access: | http://journalarticle.ukm.my/11786/1/44_4_03.pdf http://journalarticle.ukm.my/11786/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=554&catid=59:current-view&Itemid=56 |
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