Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase
BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chernically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-P...
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| Main Authors: | , |
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| 格式: | Article |
| 語言: | English |
| 出版: |
John Wiley & Sons Inc.
2008
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| 主題: | |
| 在線閱讀: | http://eprints.utm.my/id/eprint/12517/1/FuiLingWong2008_JournalofChemicalTechnologyandBiotechnology.pdf http://eprints.utm.my/id/eprint/12517/ http://dx.doi.org/10.1002/jctb.1774 |
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| 總結: | BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chernically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-PVA hybrid material.
RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross-linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of K-m(app).
CONCLUSION: Freeze-thawed PVA-GOD membranes and TMOS-PVA, which showed satisfactory sensitivity and adequate value of K-m(app), were quite promising as support materials for immobilizing GOD. (c) 2007 Society of Chemical Industry. |
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