Process optimization of glucose oxidase production by using locally isolated fungal strain
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Universiti Malaysia Perlis
2011
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my.unimap-133532011-07-30T08:21:29Z Process optimization of glucose oxidase production by using locally isolated fungal strain Adib Zubaidi, Rashid Assoc. Prof. Dr. Dachyar Arbain (Advisor) Glucose oxidase Aspergillus terreus Enzymes Fungal strain Access is limited to UniMAP community. Glucose oxidase (β-D-glucose:oxygen 1-oxidoreductase; EC 1.1.2.3.4) catalyzes the oxidation of β-D-glucose to gluconic acid, by utilizing molecular oxygen as an electron acceptor with simultaneous production of hydrogen peroxide. Effects of pH, temperature and agitation on the production of glucose oxidase from Aspergillus terreus were studied. The process conditions were optimized by using Central Composite Design approach. The experimental data obtained were fitted to a second–order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. Glucose oxidase with the activity of 7.391 U/ml was produced at pH 6.5, 35 oC and 250 rpm as the optimum conditions. Under these conditions, the experimental percentage error was 1.02 %, showing a close agreement with value predicted by the model. 2011-07-30T08:21:29Z 2011-07-30T08:21:29Z 2011-05 Learning Object http://hdl.handle.net/123456789/13353 en Universiti Malaysia Perlis School of Bioprocess Engineering |
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Universiti Malaysia Perlis |
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UniMAP Library |
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Institutional Repository |
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Asia |
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Malaysia |
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Universiti Malaysia Perlis |
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UniMAP Library Digital Repository |
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http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
Glucose oxidase Aspergillus terreus Enzymes Fungal strain |
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Glucose oxidase Aspergillus terreus Enzymes Fungal strain Adib Zubaidi, Rashid Process optimization of glucose oxidase production by using locally isolated fungal strain |
| description |
Access is limited to UniMAP community. |
| author2 |
Assoc. Prof. Dr. Dachyar Arbain (Advisor) |
| author_facet |
Assoc. Prof. Dr. Dachyar Arbain (Advisor) Adib Zubaidi, Rashid |
| format |
Learning Object |
| author |
Adib Zubaidi, Rashid |
| author_sort |
Adib Zubaidi, Rashid |
| title |
Process optimization of glucose oxidase production by using locally isolated fungal strain |
| title_short |
Process optimization of glucose oxidase production by using locally isolated fungal strain |
| title_full |
Process optimization of glucose oxidase production by using locally isolated fungal strain |
| title_fullStr |
Process optimization of glucose oxidase production by using locally isolated fungal strain |
| title_full_unstemmed |
Process optimization of glucose oxidase production by using locally isolated fungal strain |
| title_sort |
process optimization of glucose oxidase production by using locally isolated fungal strain |
| publisher |
Universiti Malaysia Perlis |
| publishDate |
2011 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/13353 |
| _version_ |
1643790816007684096 |
| score |
13.244413 |