Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli

Mangifera caesia also known as binjai, is one of the medicinal plant that used by people of Sabah particularly in Papar and Kota Belud districts to increase appetite, treating cold, body itchiness, high blood pressure, and bronchitis. There has been no report regarding the antimitotic potential of t...

全面介绍

Saved in:
书目详细资料
主要作者: Rusli, Muhammad Fadzli
格式: Thesis
语言:English
出版: 2019
主题:
在线阅读:https://ir.uitm.edu.my/id/eprint/50565/1/50565.pdf
https://ir.uitm.edu.my/id/eprint/50565/
标签: 添加标签
没有标签, 成为第一个标记此记录!
id my.uitm.ir.50565
record_format eprints
spelling my.uitm.ir.505652021-09-15T09:18:07Z https://ir.uitm.edu.my/id/eprint/50565/ Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli Rusli, Muhammad Fadzli Botanical chemistry. Phytochemicals Individual drugs and other agents Fruit and fruit culture Mangifera caesia also known as binjai, is one of the medicinal plant that used by people of Sabah particularly in Papar and Kota Belud districts to increase appetite, treating cold, body itchiness, high blood pressure, and bronchitis. There has been no report regarding the antimitotic potential of the plant. The aim of this study is to assess the M. caesia of its mitodepressive effect against dividing cell. Allium cepa root was tested against the aqueous and methanol extract of binjai for 24 hours. The root of Allium cepa were treated with different concentration (4-6 mg/mL) of M. caesia aqueous fruit extract (MCAE) and M. caesia methanol fruit extract (MCME), distilled water was used as negative control and sodium azide (0.1 mg/mL) was used as positive control. The cells then crushed and stain with 0.5% methylene blue and observed microscopically to calculate the mean percentage of the Mitotic Index (MI). MCAE with concentration of 6 mg/mL was found to significantly (p=0.013) reduced the MI of Allium cepa compared to the negative control (distilled water). Similarly, MCAE at 5 mg/mL was also found to have significant (p=0.048) reduction of MI. In comparison, only MCME with concentration of 6 mg/mL found to significantly (p=0.009) reduced the MI of Allium cepa. Increase MI reduction with the increase of extract concentration suggest a dose dependent effect. The result indicate cytotoxic potential of both MCAE and MCME due to the significant mitodepressive effect. In conclusion, both MCAE and MCME exhibits antimitotic activity against Allium cepa. The study give credence to medicinal potential of this plant and need to conduct further sophisticated test to determine the anticipated anticancer potential. 2019 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50565/1/50565.pdf ID50565 Rusli, Muhammad Fadzli (2019) Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli. Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Botanical chemistry. Phytochemicals
Individual drugs and other agents
Fruit and fruit culture
spellingShingle Botanical chemistry. Phytochemicals
Individual drugs and other agents
Fruit and fruit culture
Rusli, Muhammad Fadzli
Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli
description Mangifera caesia also known as binjai, is one of the medicinal plant that used by people of Sabah particularly in Papar and Kota Belud districts to increase appetite, treating cold, body itchiness, high blood pressure, and bronchitis. There has been no report regarding the antimitotic potential of the plant. The aim of this study is to assess the M. caesia of its mitodepressive effect against dividing cell. Allium cepa root was tested against the aqueous and methanol extract of binjai for 24 hours. The root of Allium cepa were treated with different concentration (4-6 mg/mL) of M. caesia aqueous fruit extract (MCAE) and M. caesia methanol fruit extract (MCME), distilled water was used as negative control and sodium azide (0.1 mg/mL) was used as positive control. The cells then crushed and stain with 0.5% methylene blue and observed microscopically to calculate the mean percentage of the Mitotic Index (MI). MCAE with concentration of 6 mg/mL was found to significantly (p=0.013) reduced the MI of Allium cepa compared to the negative control (distilled water). Similarly, MCAE at 5 mg/mL was also found to have significant (p=0.048) reduction of MI. In comparison, only MCME with concentration of 6 mg/mL found to significantly (p=0.009) reduced the MI of Allium cepa. Increase MI reduction with the increase of extract concentration suggest a dose dependent effect. The result indicate cytotoxic potential of both MCAE and MCME due to the significant mitodepressive effect. In conclusion, both MCAE and MCME exhibits antimitotic activity against Allium cepa. The study give credence to medicinal potential of this plant and need to conduct further sophisticated test to determine the anticipated anticancer potential.
format Thesis
author Rusli, Muhammad Fadzli
author_facet Rusli, Muhammad Fadzli
author_sort Rusli, Muhammad Fadzli
title Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli
title_short Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli
title_full Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli
title_fullStr Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli
title_full_unstemmed Assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / Muhammad Fadzli Rusli
title_sort assessment of antimitotic activity of mangifera caesia (binjai) fruit extract using allium cepa test / muhammad fadzli rusli
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/50565/1/50565.pdf
https://ir.uitm.edu.my/id/eprint/50565/
_version_ 1712288395954225152
score 13.209306