Characterization and solvent stability of strep-tagged purified recombinant lipase from thermostable and solvent tolerant Bacillus sp. strain 42.

• المؤلفون الرئيسيون: Tengku Abdul Hamid, Tengku Haziyamin, Mohamed , Abdallah Eltaweel, Raja Abdul Rahman, Raja Noor Zaliha, Basri, Mahiran, Salleh, Abu Bakar
• التنسيق: مقال
• اللغة: English
• منشور في: Springer 2009
• الموضوعات: Q Science (General)
• الوصول للمادة أونلاين: http://irep.iium.edu.my/1558/1/Tg_Annal-Micribiology.pdf; http://irep.iium.edu.my/1558/; http://www.springerlink.com/content/r69471q47k057653/

A 1.2 kb lipase gene (AY 78735) from solvent stable and thermostable Bacillus sp. strain 42 was overexpressed in a heterologous system that allowed for an extensive characterization of its solvent stability and thermostability. An overexpression was achieved using pET51b vector with Escherichia col...