Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium
Present investigation deals with optimization of appropriate substrate concentration and incubation temperature both for growth of Saccharomyces cerevisiae and invertase production. Submerged fermentation technique was employed in the present study. The maximal production of invertase during the cou...
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| Language: | en |
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2006
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| Online Access: | http://eprints.utm.my/183/1/IkramulHaq2006_Kineticsofinvertasesynthesisby.pdf http://eprints.utm.my/183/ |
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| author | Ikram-ul-Haq, Ikram-ul-Haq Mukhtar, Hamid |
| author_facet | Ikram-ul-Haq, Ikram-ul-Haq Mukhtar, Hamid |
| author_sort | Ikram-ul-Haq, Ikram-ul-Haq |
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| content_provider | Universiti Teknologi Malaysia |
| content_source | UTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Present investigation deals with optimization of appropriate substrate concentration and incubation temperature both for growth of Saccharomyces cerevisiae and invertase production. Submerged fermentation technique was employed in the present study. The maximal production of invertase during the course of study was achieved after 48 h of incubation using initial sucrose concentration, 15.0 g l-1. The sugar consumption and dry cell mass were also examined. Data was subjected to kinetic analysis and on the basis of kinetic parameters such as Yp/x (amount of enzyme produced mg-1 cell mass), Yp/s (amount of enzyme produced mg-1 sugar consumed), Yx/s (mg cells mg-1 substrate consumed), Ys/x (mg sugar consumed mg-1 cell mass produced), qp (Amount of enzyme produced mg-1 sugar consumed h-1), qs (mg mg-1 cells h-1), qx (mg cells mg-1 sugar consumed h-1), µ (mg cells produced h-1), it was found that temperature had a direct influence both on substrate consumption and synthesis of enzyme. Similarly, higher concentrations of sucrose in fermentation medium induced catabolite repression of yeast invertase. |
| format | Conference or Workshop Item |
| id | my.utm.eprints-183 |
| institution | Universiti Teknologi Malaysia |
| language | en |
| publishDate | 2006 |
| record_format | eprints |
| spelling | my.utm.eprints-1832017-08-23T08:02:45Z http://eprints.utm.my/183/ Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium Ikram-ul-Haq, Ikram-ul-Haq Mukhtar, Hamid TP Chemical technology Present investigation deals with optimization of appropriate substrate concentration and incubation temperature both for growth of Saccharomyces cerevisiae and invertase production. Submerged fermentation technique was employed in the present study. The maximal production of invertase during the course of study was achieved after 48 h of incubation using initial sucrose concentration, 15.0 g l-1. The sugar consumption and dry cell mass were also examined. Data was subjected to kinetic analysis and on the basis of kinetic parameters such as Yp/x (amount of enzyme produced mg-1 cell mass), Yp/s (amount of enzyme produced mg-1 sugar consumed), Yx/s (mg cells mg-1 substrate consumed), Ys/x (mg sugar consumed mg-1 cell mass produced), qp (Amount of enzyme produced mg-1 sugar consumed h-1), qs (mg mg-1 cells h-1), qx (mg cells mg-1 sugar consumed h-1), µ (mg cells produced h-1), it was found that temperature had a direct influence both on substrate consumption and synthesis of enzyme. Similarly, higher concentrations of sucrose in fermentation medium induced catabolite repression of yeast invertase. 2006-07 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/183/1/IkramulHaq2006_Kineticsofinvertasesynthesisby.pdf Ikram-ul-Haq, Ikram-ul-Haq and Mukhtar, Hamid (2006) Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia. |
| spellingShingle | TP Chemical technology Ikram-ul-Haq, Ikram-ul-Haq Mukhtar, Hamid Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium |
| title | Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium |
| title_full | Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium |
| title_fullStr | Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium |
| title_full_unstemmed | Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium |
| title_short | Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium |
| title_sort | kinetics of invertase synthesis by saccharomyces cerevisiae in synthetic medium |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/183/1/IkramulHaq2006_Kineticsofinvertasesynthesisby.pdf http://eprints.utm.my/183/ |
| url_provider | http://eprints.utm.my/ |