Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR
This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive...
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| Format: | Article |
| Language: | en |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52353/1/Detection%20of%20Vibrio%20parahaemolyticus%20in%20cockle%20%28Anadara%20granosa%29%20by%20PCR.pdf http://psasir.upm.edu.my/id/eprint/52353/ http://onlinelibrary.wiley.com/wol1/doi/10.1016/j.femsle.2005.08.053/abstract |
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| author | Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Wong, Clemente Michael Vui Ling Tanil, Gwendelynne Bulan Nishibuchi, Mitsuaki |
| author_facet | Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Wong, Clemente Michael Vui Ling Tanil, Gwendelynne Bulan Nishibuchi, Mitsuaki |
| author_sort | Bilung, Lesley Maurice |
| building | UPM Library |
| collection | Institutional Repository |
| content_provider | Universiti Putra Malaysia |
| content_source | UPM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption. |
| format | Article |
| id | my.upm.eprints-52353 |
| institution | Universiti Putra Malaysia |
| language | en |
| publishDate | 2005 |
| publisher | Elsevier |
| record_format | eprints |
| spelling | my.upm.eprints-523532017-06-05T09:34:13Z http://psasir.upm.edu.my/id/eprint/52353/ Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Wong, Clemente Michael Vui Ling Tanil, Gwendelynne Bulan Nishibuchi, Mitsuaki This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption. Elsevier 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52353/1/Detection%20of%20Vibrio%20parahaemolyticus%20in%20cockle%20%28Anadara%20granosa%29%20by%20PCR.pdf Bilung, Lesley Maurice and Radu, Son and Bahaman, Abdul Rani and Abdul Rahim, Raha and Napis, Suhaimi and Wong, Clemente Michael Vui Ling and Tanil, Gwendelynne Bulan and Nishibuchi, Mitsuaki (2005) Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR. FEMS Microbiology Letters, 252 (1). pp. 85-88. ISSN 0378-1097; ESSN: 1574-6968 http://onlinelibrary.wiley.com/wol1/doi/10.1016/j.femsle.2005.08.053/abstract 10.1016/j.femsle.2005.08.053 |
| spellingShingle | Bilung, Lesley Maurice Radu, Son Bahaman, Abdul Rani Abdul Rahim, Raha Napis, Suhaimi Wong, Clemente Michael Vui Ling Tanil, Gwendelynne Bulan Nishibuchi, Mitsuaki Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_full | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_fullStr | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_full_unstemmed | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_short | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_sort | detection of vibrio parahaemolyticus in cockle (anadara granosa) by pcr |
| url | http://psasir.upm.edu.my/id/eprint/52353/1/Detection%20of%20Vibrio%20parahaemolyticus%20in%20cockle%20%28Anadara%20granosa%29%20by%20PCR.pdf http://psasir.upm.edu.my/id/eprint/52353/ http://onlinelibrary.wiley.com/wol1/doi/10.1016/j.femsle.2005.08.053/abstract |
| url_provider | http://psasir.upm.edu.my/ |