Halal Process of Newcastle Disease Vaccine Production in Bioreactor

• Main Author: A. Mohd Azmir Arifin
• Format: Conference Paper
• Language: English
• Published: 2013
• Subjects: Vaccine > Halal process > Bioreactor
• Online Access: http://ddms.usim.edu.my/handle/123456789/6124

Nowadays, halal concept is beginning to be more appreciated throughout the world. The acceptance of the halal concept is due to hygiene, sanitation and safety aspects that are much emphasized while fulfilling the shariah requirement at the same time. In this study, we have developed a halal process for Newcastle disease vaccine production using cell culture in bioreactor. The process developed can serve as platform for production of other halal vaccines because it is not just safe and hygiene but also non-halal sources free. Firstly, selected strain of Newcastle disease virus is adapted to several cell lines. Secondly, medium for cell and virus culture is optimized to generate high virus titer. Later, the process is scaled up from T-flask to spinner vessel and stirred tank bioreactor culture and optimized for mass production of the virus. Throughout the whole process, porcine derived trypsin is replaced with accutase enzyme which is derived from invertebrate sources. In addition, Cytodex 1 microcarriers that is used in bioreactor culture is porcine free and attracts the cells by having positive charge. As a conclusion. the process developed for the vaccine production is halal as it is porcine free and very efficient, safer, and more hygiene compared to the traditional method which uses embryonated chicken eggs-