Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis
A recent upsurge in ocular infections is a pointer towards an enhanced prevalence of ophthalmic disorders, posing challenges for researchers globally. The caveats of conventional therapeutics demand a specifically designed Ocular Drug Delivery System (ODDS) and hence the primary objective of the pre...
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my.ump.umpir.355502022-11-03T03:55:07Z http://umpir.ump.edu.my/id/eprint/35550/ Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology A recent upsurge in ocular infections is a pointer towards an enhanced prevalence of ophthalmic disorders, posing challenges for researchers globally. The caveats of conventional therapeutics demand a specifically designed Ocular Drug Delivery System (ODDS) and hence the primary objective of the present work is a fabrication of a Design of Expert (DoE) guided Chitosan based Antifungal loaded Nanoparticles (CANs), as a locoregionally effective eye formulation/drops for fungal keratitis therapy. The purported formulation was prepared using High-Pressure Homogenisation technique and was critically characterized on various parameters to check their suitability as an ODDS. The optimized formulation has fruitfully yielded irregularly spherical particles in up to a size of 200 nm and a Poly-dispersity Index (PDI) of less than 0.2 nm. The optimised formulation has further showcased a high mucoadhesion capacity thereby, suggesting the greater retention of CANs on the mucous membrane of an eye with low ocular irritancy as highlighted using HET-CAM (Hen's Egg Chorioallantoic Membrane) test. The in-vitro drug release study across a dialysis membrane has indicated both diffusion as swelling controlled release pattern for an optimized formulation. The ex-vivo corneal permeation study on goat corneal tissues using a Franz-Diffusion cell also has indicated a steady increase in the permeation of drug with time for an optimized formulation. Further, the optimised formulation was found to be non-irritant and ocular safe in ex-vivo transcorneal toxicity studies on goat corneal tissues. In conclusion, the designing of a proposed nanosized formulation, offers a promising step towards the management of external ocular diseases with a positive attributes of high patient compliance, controlled drug delivery, prolonged drug precorneal residence time and enhanced ocular bioavailability. The optimized CANs could be further exploited as a potential ODDS. Elsevier Ltd 2020 Conference or Workshop Item PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/35550/1/Doe%20guided%20chitosan%20based%20nano-ophthalmic%20preparation%20against%20fungal%20keratitis.pdf Hassan, Nazia and Mirza, Mohd Aamir and Aslam, Mohammed and Mahmood, Syed and Iqbal, Zeenat (2020) Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis. In: Materials Today: Proceedings, 11th Malaysian Technical Universities Conference on Engineering & Technology, 19-22 November 2019 , Bukit Gambang Resort City, Pahang, Malaysia. pp. 19-29., 41 (Part 1). ISSN 2214-7853 https://doi.org/10.1016/j.matpr.2020.09.823 |
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QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
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A recent upsurge in ocular infections is a pointer towards an enhanced prevalence of ophthalmic disorders, posing challenges for researchers globally. The caveats of conventional therapeutics demand a specifically designed Ocular Drug Delivery System (ODDS) and hence the primary objective of the present work is a fabrication of a Design of Expert (DoE) guided Chitosan based Antifungal loaded Nanoparticles (CANs), as a locoregionally effective eye formulation/drops for fungal keratitis therapy. The purported formulation was prepared using High-Pressure Homogenisation technique and was critically characterized on various parameters to check their suitability as an ODDS. The optimized formulation has fruitfully yielded irregularly spherical particles in up to a size of 200 nm and a Poly-dispersity Index (PDI) of less than 0.2 nm. The optimised formulation has further showcased a high mucoadhesion capacity thereby, suggesting the greater retention of CANs on the mucous membrane of an eye with low ocular irritancy as highlighted using HET-CAM (Hen's Egg Chorioallantoic Membrane) test. The in-vitro drug release study across a dialysis membrane has indicated both diffusion as swelling controlled release pattern for an optimized formulation. The ex-vivo corneal permeation study on goat corneal tissues using a Franz-Diffusion cell also has indicated a steady increase in the permeation of drug with time for an optimized formulation. Further, the optimised formulation was found to be non-irritant and ocular safe in ex-vivo transcorneal toxicity studies on goat corneal tissues. In conclusion, the designing of a proposed nanosized formulation, offers a promising step towards the management of external ocular diseases with a positive attributes of high patient compliance, controlled drug delivery, prolonged drug precorneal residence time and enhanced ocular bioavailability. The optimized CANs could be further exploited as a potential ODDS. |
format |
Conference or Workshop Item |
author |
Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat |
author_facet |
Hassan, Nazia Mirza, Mohd Aamir Aslam, Mohammed Mahmood, Syed Iqbal, Zeenat |
author_sort |
Hassan, Nazia |
title |
Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
title_short |
Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
title_full |
Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
title_fullStr |
Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
title_full_unstemmed |
Doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
title_sort |
doe guided chitosan based nano-ophthalmic preparation against fungal keratitis |
publisher |
Elsevier Ltd |
publishDate |
2020 |
url |
http://umpir.ump.edu.my/id/eprint/35550/1/Doe%20guided%20chitosan%20based%20nano-ophthalmic%20preparation%20against%20fungal%20keratitis.pdf http://umpir.ump.edu.my/id/eprint/35550/ https://doi.org/10.1016/j.matpr.2020.09.823 |
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1748703319798317056 |
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13.1944895 |